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The use of self-interaction chromatography in stable formulation and crystallization of proteins

✍ Scribed by Kedar S. Deshpande; Tangir Ahamed; Joop H. ter Horst; Peter J. Jansens; Luuk A. M. van der Wielen; Marcel Ottens


Book ID
112011781
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
442 KB
Volume
4
Category
Article
ISSN
1860-6768

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Isocratic hydrophobic interaction chromatography of five proteins has been carried out using mobile phases containing the surfactant 3-(3-cholamidopropyl)dimethylammoniopropane sulfonate (CHAPS). Linear relationships were found between log k' and ammonium sulfate concentrations for all the proteins