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The Use of RP-HPLC for measuring activation and cleavage of rFVIIa during purification

✍ Scribed by Inger Mollerup; Stephen Bayne; Erik Halkjaer; Tony Jørgensen; Gitte Mandrup; Else Marie Nicolaisen; Lars Thim

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
403 KB
Volume
48
Category
Article
ISSN
0006-3592

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✦ Synopsis

A reverse phase HPLC (RP-HPLC) method for analysis of recombinant factor Vlla (rFVlla) has been developed. The method discriminates between different forms of recombinant FVll (rFVII). To obtain separation of these closely related molecules the method has been optimized with respect to gradient profile and temperature. The method has been used for optimization of purification processes and for kinetic studies. Evidence for autolytic cleavage was obtained.

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