Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode
✍ Scribed by Hui Zhang; Zhimou Guo; Wei Li; Jiatao Feng; Yuansheng Xiao; Feifang Zhang; Xingya Xue; Xinmiao Liang
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 824 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A four‐channel preparative HPLC was employed to isolate and purify compounds from licorice extract. Two separation modes, RP and hydrophilic interaction LC (HILIC), were used in preparative HPLC. HILIC mode was adopted to resolve the purification of the compounds with similar hydrophobicity, which were co‐eluted under RP mode. Using the two separation modes during the purification process, fifteen compounds were isolated from licorice extract. The results indicated that preparative HPLC performed under HILIC mode is an efficient method for the isolation and purification of compounds from natural products.