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The use of polyacrylamide gel electrophoresis for the analysis of acidic glycans labeled with the fluorophore 2-aminoacridone

โœ Scribed by Dr. Peter Jackson; Malcolm G. Pluskal; William Skea

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
727 KB
Volume
15
Category
Article
ISSN
0173-0835

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Numerous monosaccharides and oligosaccharides were derivatized at their reducing end groups with the fluorophore 2-aminoacridone. The resulting fluorescent compounds were separated by PAGE using two different buffer systems. One of these, a Tris borate buffer, enabled all of the fluorescent sacchari

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Insulin, hemoglobin, egg albumin, gamma globulin, bovine serum albumin, and phosphorylase a were separated by SDS-polyacrylamide gel electrophoresis, and continuously recovered for quantitation in aqueous solution using a new technique. The proteins, which were eluted in the order of their molecular