๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Quantitative analysis of proteins by the use of SDS-polyacrylamide-gel electrophoresis

โœ Scribed by Tezro Asao

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
547 KB
Volume
77
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

Insulin, hemoglobin, egg albumin, gamma globulin, bovine serum albumin, and phosphorylase a were separated by SDS-polyacrylamide gel electrophoresis, and continuously recovered for quantitation in aqueous solution using a new technique. The proteins, which were eluted in the order of their molecular weight, were determined by their absorbance at 200 nm. The recovery of the eluted protein fractions was virtually complete.

๐Ÿ“œ SIMILAR VOLUMES

Isoelectric focusing of proteins separat
Isoelectric focusing of proteins separated by SDS-polyacrylamide gel electrophoresis
โœ Gen-Ichi Danno ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 779 KB

A rapid and simple method for isoelectric focusing of proteins separated by SDS-polyacrylamide gel electrophoresis was described. The focusing system was composed of a polyacrylamide gel layer (7 cm in height) and a solution layer (1.2 cm in height) superimposed on the gel layer. Both layers contain

Recovery of SDS-proteins from polyacryla
Recovery of SDS-proteins from polyacrylamide gels by electrophoresis into hydroxylapatite
โœ Barry R. Ziola; Douglas G. Scraba ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 394 KB

A method is described which permits the purification of proteins in quantities necessary for physicochemical characterization. The protein to be purified is separated from contaminants by electrophoresis in a number of sodium dodecyl sulfate (SDS)-containing polyacrylamide disc gels. The region of e