The Use of Liposomes to Study COPII- and COPI-Coated Vesicle Formation and Membrane Protein Sorting
β Scribed by Ken Matsuoka; Randy Schekman
- Book ID
- 115639146
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 561 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1046-2023
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Vesicles having diameters from 20 to 200 nm were prepared from egg-yolk phosphatidylcholine (PC) and were separated as well as analyzed by methods that can be carried out with standard laboratory equipment. Gel-chromatography on Sephacryl S 1000 was adapted for expeditious size analysis of vesicles
## Abstract Incubation of placental brush border membrane (BBM) along with sonicated vesicles of exogenous lipids (egg yolk PC) in the presence of phospholipidβtransfer protein (PLβTP) showed a decrease in the alkaline phosphatase activity due to the change in the membrane microβenvironment, such a