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The use of formamide gradients to distinguish between alkali labile regions and single strand breaks in DNA

✍ Scribed by David Gaudin; K.Lemone Yielding


Book ID
118853032
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
266 KB
Volume
47
Category
Article
ISSN
0006-291X

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Determination of DNA single-strand break
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The detection of DNA single-strand breaks (SSB) in human mononucleated white blood cells (MWBC) using a modified version of the nick translation assay is presented. This assay allows rapid and sensitive examination of SSB using only 5 ml heparinized blood fo'~ an eightfold determination. The assay w