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The use of dilution rate cycling to stabilise recombinant plasmids in continuous culture of recombinant Saccharomyces cerevisiae

✍ Scribed by Khaswar Syamsu; Paul F. Greenfield; David A. Mitchell


Book ID
119132282
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
492 KB
Volume
45
Category
Article
ISSN
0168-1656

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Overexpression of the homologous protein proteinase A (PrA) in Saccharomyces cerevisiae has been achieved by inserting the PrA gene (PEP4) with its own promoter on a 2Β΅ multicopy plasmid. With this system the specific PrA production rate was found to be described well by a linear function of the oxi