𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The use of bioluminescence to study the behaviour of Listeria monocytogenes when attached to surfaces

✍ Scribed by Athene J. Walker; John T. Holah; Stephen P. Denyer; Gordon S.A.B. Stewart


Book ID
103966761
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
396 KB
Volume
77
Category
Article
ISSN
0927-7757

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✦ Synopsis


A recombinant

derivative of Listeria monocytogenes engineered to express the luxAi3 genes of Vibrio harueyi has a bioluminescent phenotype that provides a rapid monitor of microbial viability. This phenotype was employed to follow bacterial attachment on to stainless steel surfaces, to monitor the growth of microcolonies upon these surfaces and to assess the resistance of the attached cells following biocide challenge.

Real-time bioluminescence measurements provided data equivalent to viable count determinations, and the organism was found to become more resistant to the biocide phenol upon attachment to stainless steel surfaces.


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