✦ LIBER ✦

The Use of Automated Solid Phase Extraction in the '96 well' Format for High Throughput Bioanalysis using Liquid Chromatography Coupled to Tandem Mass Spectrometry

✍ Scribed by John P. Allanson; Robert A. Biddlecombe; Anne E. Jones; Stephen Pleasance

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 618 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199605)10:7<811::aid-rcm561>3.0.co;2-q

No coin nor oath required. For personal study only.

✦ Synopsis

A high throughput mass spectrometry based method is described for the determination of the 5-HT receptor agonist 31100, and its desmethyl metabolite, in human plasma. Samples were extracted using the MicroLutem system of solid phase extraction in the '96 well' format, automated by means of a robotic sample processor. The extracts were analysed by liquid chromatography tandem mass spectrometry (LC/MS/MS) with thermally assisted electrospray ionization (TbrboIonSpray) and selected-reaction monitoring. The LCIMSNS method offers increased sensitivity, selectivity and speed of analysis over an existing high performance liquid chromotographic method using fluorescence detection.

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