The Use of an Ethidium Analogue in the Dye-Buoyant Density Procedure for the Isolation of Closed Circular DNA: The Variation of the Superhelix Density of Mitochondrial DNA
β Scribed by Bruce Hudson, William B. Upholt, Joseph Devinny and Jerome Vinograd
- Book ID
- 123648868
- Publisher
- National Academy of Sciences
- Year
- 1969
- Tongue
- English
- Weight
- 1021 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/59178
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A simple method for directly dete~in~ng the superhelix density of closed circular DNAs by ethidium bromide titration using agarose ge1 electrophoresis in tubes containing increasing concentrations of the dye has been deveIoped. Since the positively charged dye migrates toward the cathode in a direct
A method is described for the preparative purification of supercoiled DNA of bacterial cell extracts without an ultracentrifugal step. DNA of crude cell lysates, containing supercoiled plasmid DNA and chromosomal DNA fragments is freed of protein by chromatography on Sepharose B4. The separation of