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A Dye-Buoyant-Density Method for the Detection and Isolation of Closed Circular Duplex DNA: The Closed Circular DNA in Hela Cells

โœ Scribed by Roger Radloff, William Bauer and Jerome Vinograd


Book ID
123647800
Publisher
National Academy of Sciences
Year
1967
Tongue
English
Weight
1020 KB
Volume
57
Category
Article
ISSN
0027-8424

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โœ Romilio T. Espejo; Jacob Lebowitz ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 590 KB

A simple method for directly dete~in~ng the superhelix density of closed circular DNAs by ethidium bromide titration using agarose ge1 electrophoresis in tubes containing increasing concentrations of the dye has been deveIoped. Since the positively charged dye migrates toward the cathode in a direct