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The Use of Aequorin and Its Variants for Ca2+ Measurements

โœ Scribed by Granatiero, V.; Patron, M.; Tosatto, A.; Merli, G.; Rizzuto, R.

Book ID
123613224
Publisher
Cold Spring Harbor Laboratory Press
Year
2013
Tongue
English
Weight
412 KB
Volume
2014
Category
Article
ISSN
1940-3402

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We assessed the possible effects of the volatile halogenated anesthetics halothane, enflurane, and isoflurane on Ca 2ุ‰ electrode measurements and on the Ca 2ุ‰ sensitivity of the bioluminescent protein aequorin. In Ca 2ุ‰ -EGTA buffers of different pCa values (7.870, 6.726, 6.033, 4.974, 4.038, and 2.