Nuclear targeting of aequorin: A new approach for measuring nuclear Ca2+ concentration in intact cells
โ Scribed by M Brini; R Marsault; C Bastianutto; T Pozzan; R Rizzuto
- Book ID
- 113236692
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 927 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0143-4160
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In the last decade, the study of Ca 2ฯฉ homeostasis within organelles in living cells has been greatly enhanced by the utilisation of a recombinant Ca 2ฯฉ -sensitive photoprotein, aequorin. Aequorin is a Ca 2ฯฉ sensitive photoprotein of a coelenterate that, in the past, was widely employed to measure C
## Abstract Calciumโbinding protein 1 (CaBP1), a neuronโspecific member of the calmodulin (CaM) superfamily, regulates the Ca^2+^โdependent activity of inositol 1,4,5โtriphosphate receptors (InsP3Rs) and various voltageโgated Ca^2+^ channels. Here, we present the NMR structure of fullโlength CaBP1