Intracellular targeting of the photoprotein aequorin: A new approach for measuring, in living cells, Ca2+concentrations in defined cellular compartments

Targeted recombinant aequorins: Tools fo

Targeted recombinant aequorins: Tools for monitoring [Ca2+] in the various compartments of a living cell

✍ Brini, Marisa; Pinton, Paolo; Pozzan, Tullio; Rizzuto, Rosario 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 244 KB 👁 1 views

In the last decade, the study of Ca 2ϩ homeostasis within organelles in living cells has been greatly enhanced by the utilisation of a recombinant Ca 2ϩ -sensitive photoprotein, aequorin. Aequorin is a Ca 2ϩ sensitive photoprotein of a coelenterate that, in the past, was widely employed to measure C