The use of a tumor promoter for the detection of individuals with the gardner syndrome

✍ Scribed by Levy Kopelovich; Eldon J. Gardner

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 422 KB
• Volume: 51
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19830215)51:4<716::aid-cncr2820510427>3.0.co;2-s

✦ Synopsis

The effects of the tumor promoter 12-0-tetradecnoyl phorbol-13-acetate (TPA) on the proliferation out of cultured skin fibroblasts (SF) obtained from 21 individuals representing a single pedigree of the Gardner variant of hereditary adenomatosis of the colon and rectum (ACR) were analyzed. SF from both gene-carriers and normal individuals displayed an unusual biphasic dose-response (concaved upward), but the latter were considerably more sensitive to the toxic effects of this probe a t all concentrations tested. Based on the differential sensitivity to TPA (range, 0-100 ng/ml),'*' a good correlation has been found in this study between the results, the pedigree analysis, and the clinical findings. Of 21 individuals examined, two were apparently false-negatives. Two other individuals who are currently listed as clinically asymptomatic and, who through pedigree analysis might presumably be disease-free, appeared strongly positive by the criteria. The results extend the previous observations that the measurement of cloning efficiency in the presence of a TPA probe provides a reliable assay to distinguish SF of colorectal cancer-prone persons from those of normal subjects within a single pedigree of the Gardner syndrome.