𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The use of a novel plate assay in a search for yeast mutants defective in deoxyribonucleases

✍ Scribed by Johnston, Leland H.

Publisher
Springer
Year
1977
Tongue
English
Weight
387 KB
Volume
152
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Methods by which the intracellular enzymes deoxyribonuclease, ribonuclease and protease can be assayed in whole colonies of Saccharomyces cerevisiae on agar plates are described. A search for mutants deficient in deoxyribonuclease has been carried out. Two types of mutant are described. One apparently fails to produce deoxyribonuclease, ribonuclease or protease on agar plates and the other apparently fails to produce deoxyribonuclease and ribonuclease.

πŸ“œ SIMILAR VOLUMES

A yeast mutant defective in the processi
A yeast mutant defective in the processing of 27S r-RNA precursor
✍ Andrew, Curtis ;Hopper, Anita K. ;Hall, Benjamin D. πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 853 KB

Among a group of 31 ts- yeast mutants screened electrophoretically for heat-sensitive synthesis of each stable RNA species, only mutant ts351 failed to accumulate 25S RNA at 36 degrees C. Pulse-labeling experiments at 36 degrees C showed that 35S and 27S precursors RNA and mature 18S r-RNA molecules

A new method for quantitative determinat
A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants ofSaccharomyces cerevisiae
✍ Dallies, Nathalie; FranΓ§ois, Jean; Paquet, Veronique πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 148 KB πŸ‘ 3 views

A reliable acid hydrolysis method for quantitative determination of the proportion of -glucan, mannan and chitin in Saccharomyces cerevisiae cell wall is reported together with a simple extraction procedure to quantify within a standard error of less than 2% the proportion of the wall per gram of ce