A yeast mutant defective in the processing of 27S r-RNA precursor

✍ Scribed by Andrew, Curtis ;Hopper, Anita K. ;Hall, Benjamin D.

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 853 KB
• Volume: 144
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00277300

✦ Synopsis

Among a group of 31 ts- yeast mutants screened electrophoretically for heat-sensitive synthesis of each stable RNA species, only mutant ts351 failed to accumulate 25S RNA at 36 degrees C. Pulse-labeling experiments at 36 degrees C showed that 35S and 27S precursors RNA and mature 18S r-RNA molecules are synthetized by ts351 cells but that 25S and 5.8S RNA species are not made and new 60S ribosomal sub-units are not assembled. The mutant is blocked at a specific point in r-RNA processing: the cutting of 27S to form 25S and 5.8S r-RNA.