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The Type I Restriction Endonuclease R.EcoR124I: Over-production and Biochemical Properties

✍ Scribed by Pavel Janscak; Agnes Abadjieva; Keith Firman


Book ID
115627322
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
617 KB
Volume
257
Category
Article
ISSN
0022-2836

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When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzy