## Abstract DNA has been isolated from human bronchial segments that have been treated in short‐term organ culture with ^3^H‐labelled benzo(a)pyrene. DNA has also been isolated from mouse skin treated with ^3^H‐labelled samples of benzo(a)pyrene, with the related radioactive 4,5‐, 7,8‐ and 9,10‐dih
The tumor promoter TPA enhances benzo[a]pyrene and benzo[a]pyrene diolepoxide mutagenesis in Big Blue® mouse skin
✍ Scribed by Marian L. Miller; Kersi Vasunia; Glenn Talaska; Anastasia Andringa; Johan de Boer; Kathleen Dixon
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 146 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0893-6692
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In the main study, tritium-labelled benzo [a]pyrene ([3H]BaP) was added to oils of a wide range of viscosity (from 13.5 to ca. 8000 cSt at 40°C) and these were applied once to mouse skin under conditions where grooming was either allowed or prevented. The binding of ['HIBaP to epidermal protein and
Big Blue@ (BB) and generic B6C3F1 mice were given one to three i.p. injections of 50 mg/kg benzo[a]pyrene (B[a]P) in DMSO every other day to achieve cumulative doses of 50 to 150 mg/kg. Three weeks after treatment, the mutation frequency at the endogenous hprt gene and lac/ transgene was measured in
## Abstract Combined subcarcinogenic doses of benzo[__a__]pyrene (BaP) and UVA induced H‐__ras,__ but not __p53,__ gene mutations 8 weeks before tumor emergence in SKH‐1 mice. Neither UVA (40 kJ/m^2^) nor BaP (8 nmol) induced any tumors after mice were topically treated 3 times/week for 25 weeks. H