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The involvement of a diol-epoxide in the metabolic activation of benzo(a)pyrene in human bronchial mucosa and in mouse skin

✍ Scribed by P. L. Grover; A. Hewer; K. Pal; P. Sims

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
569 KB
Volume
18
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

DNA has been isolated from human bronchial segments that have been treated in short‐term organ culture with ^3^H‐labelled benzo(a)pyrene. DNA has also been isolated from mouse skin treated with ^3^H‐labelled samples of benzo(a)pyrene, with the related radioactive 4,5‐, 7,8‐ and 9,10‐dihydrodiols and with ^3^H‐3‐hydroxybenzo(a)pyrene. Sephadex LH20 column chromatography of hydrolysates of these DNA samples showed that the hydrocarbon‐deoxyribonucleoside products formed in benzo(a)‐pyrene‐treated human bronchial mucosa and mouse skin are indistinguishable from those that are formed when 7,8‐dihydro‐7,8‐dihydroxybenzo(a)pyrene 9,10‐oxide reacts with DNA in solution. These same hydrocarbon‐deoxyribonucleoside products were also found in hydrolysates of DNA from mouse skin treated with 7,8‐dihydro‐7,8‐dihydroxybenzo(a)‐pyrene but products of this type were not detected in hydrolysates of DNA following treatment of mouse skin either with the 4,5‐ or 9,10‐dihydrodiols or with 3‐hydroxybenzo(a)pyrene. The results show that the metabolic activation of benzo(a)pyrene to a diolepoxide, 7,8‐dihydro‐7,8‐dihydroxybenzo(a)pyrene 9,10‐oxide, which reacts with DNA, is the same in human bronchial mucosa, a tissue in which this hydrocarbon is suspected of being carcinogenic, as it is in mouse skin and in hamster embryo cells, two situations in which benzo(a)pyrene is known to induce malignancy.

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