The ts 15 mutation of Escherichia coli alters the sequence of the C-terminal nine residues of Rho protein
β Scribed by Timothy Opperman; Asuncion Martinez; John P. Richardson
- Book ID
- 116132203
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 146 KB
- Volume
- 152
- Category
- Article
- ISSN
- 0378-1119
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A strain of Escherichia coli, VT, which spontaneously gives rise to mutations in many ribosomal proteins, has been used in conjunction with chemical mutagenesis and varying the subsequent incubation temperature to select mutants which have alterations in every ribosomal protein amenable to analysis
The plasmid pJSF6, a derivative of pBR327, could be maintained at 30 degrees C in strains of Escherichia coli containing the strong rho mutation, rho-15. Plasmids extracted from rho-15 cells were always less negatively supercoiled than plasmids from rho+ cells. Transduction experiments designed to s