𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The thioredoxin system from Streptomyces coelicolor

✍ Scribed by Petra Štefanková; Dušan Perečko; Imrich Barák; Marta Kollárová

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
119 KB
Volume
46
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

This paper describes the cloning, purification and characterization of thioredoxin (TrxA) and thioredoxin reductase (TrxR) from bacterial strain Streptomyces coelicolor . The genes of S. coelicolor encoding TrxA and TrxR were amplified by polymerase chain reaction, inserted into pET expression vector and used to overexpress these proteins in Escherichia coli . TrxA and TrxR were produced as the hexahistidine fusion proteins and were recovered from the cytoplasm as the soluble proteins. The activity of the purified recombinant proteins was demonstrated. The activity of TrxA was shown by efficient reduction of insulin and activity of NADPH‐dependent TrxR was revealed by catalyses of 5,5′‐dithiobis (2‐nitrobenzoic acid) (DTNB) reduction reaction. The reduction reaction was fully dependent upon the presence of TrxA as intermediate electron carrier with the pH optimum 7.5 and the temperature optimum 29 °C. K~m~ value of TrxR for TrxA was 0.217 ± 0.02 µM. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

📜 SIMILAR VOLUMES

The Thioredoxin System — From Science to
The Thioredoxin System — From Science to Clinic
✍ Stephan Gromer; Sabine Urig; Katja Becker 📂 Article 📅 2004 🏛 John Wiley and Sons ⚖ 50 KB

## Abstract For Abstract see ChemInform Abstract in Full Text.

The activities of the two thioredoxins f
The activities of the two thioredoxins from Streptomyces aureofaciens are not interchangeable
✍ Tatiana Horecká; Dušan Perečko; Eva Kutejová; Darina Mikulášová; Marta Kollárová 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 215 KB

The physico-chemical features of the NADPH-thioredoxin reductase (TRR) and two thioredoxins from Streptomyces aureofaciens (A14) are reported. The activity of pure S. aureofaciens thioredoxin reductase decreased drastically in the presence of NADPH or NADH while NADP(+), NAD(+), as well as S. aureof

Spontaneous mutations in the galactose o
Spontaneous mutations in the galactose operons of Streptomyces coelicolor A3 (2) and Streptomyces lividans 66
✍ Umaru Ali-Dunkrah; Kevin Kendall; Prof. Dr. John Cullum 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 403 KB

## Abstract 17 spontaneous Gal^−^ mutants of __S. coelicolor__ were studied using Southern blottings, but no insertion or deletion mutations were found. 10 mutants of __S. lividans__ included three deletion mutations. The reasons for the lack of insertion mutants are discussed.

Expression of the thioredoxin–thioredoxi
Expression of the thioredoxin–thioredoxin reductase system in the inflamed joints of patients with rheumatoid arthritis
✍ Madelon M. Maurice; Hajime Nakamura; Sonja Gringhuis; Takashi Okamoto; Shinichi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB 👁 2 views

Objective. To examine the expression of the thioredoxin (TRX)-thioredoxin reductase (TR) system in patients with rheumatoid arthritis (RA) and patients with other rheumatic diseases. Methods. Levels of TRX in plasma and synovial fluid (SF) were measured using enzyme-linked immunosorbent assay. Cell