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The Synthesis of Three AMP-Analogues: N6-(6-Aminohexyl)-adenosine 5′-Monophosphate, N6-(6-Aminohexyl)-adenosine 2′,5′-Bisphosphate, and N6-(6-Aminohexyl)-adenosine 3′,5′-Bisphosphate and Their Application as General Ligands in Biospecific Affinity Chromatography

✍ Scribed by Peter BRODELIUS; Per-Olof LARSSON; Klaus MOSBACH

Book ID: 115114284
Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 825 KB
Volume: 47
Category: Article
ISSN: 1432-1327
DOI: 10.1111/j.1432-1033.1974.tb03670.x

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Purification of malic enzyme by affinity

Purification of malic enzyme by affinity chromatography on immobilized (N6-(6-aminohexyl)-adenosine 2′,5′-bisphosphate

✍ Kwok Kam Yeung; Robert J. Carrico 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 1008 KB

A simple and rapid method for the purification of NADP-dependent malic enzyme from chicken liver is described. A crude tissue extract was chromatographed on an affinity column containing immobilized W-(6arninohexyl)adenosine 2',5'-bisphosphate. Malic enzyme bound to the ligand and was eluted specifi