The synthesis of soluble polymer-ligand complexes for affinity precipitation studies

✍ Scribed by A. P. Bradshaw; R. J. Sturgeon

• Book ID: 104647195
• Publisher: Springer-Verlag
• Year: 1990
• Tongue: English
• Weight: 303 KB
• Volume: 4
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00156613

✦ Synopsis

Protein

A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations.