Limitations of the ligand solubility method for studying complex formation
β Scribed by H.S. Rossotti
- Book ID
- 118904088
- Publisher
- Elsevier Science
- Year
- 1960
- Weight
- 209 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0022-1902
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## Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate co
Some characteristics of bi-ligand systems, important for the design and performance of studies on mixed-ligand complex formation, are given. The problem of the optimum ligand concentration is discussed using published results. In the experimental work the concentration range of the stronger complexa