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The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion

✍ Scribed by Gilbert, Michel; Bayer, Robert; Cunningham, Anna-Maria; DeFrees, Shawn; Gao, Yinghong; Watson, David C.; Young, N. Martin; Wakarchuk, Warren W.


Book ID
109907200
Publisher
Nature Publishing Group
Year
1998
Tongue
English
Weight
523 KB
Volume
16
Category
Article
ISSN
1087-0156

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✍ Franz Amann; Christoph Schaub; Bernd Müller; Richard R. Schmidt 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 286 KB 👁 2 views

Enzymatic sialyl transfer with CMP-Neu5Ac as donor can be inhibited by CDP. Therefore phosphonates 1 a,b, 2 and 3 were synthesized as substrate analogues. With a(2 ± 6)-sialyltransferase from rat liver (EC 2.4.99.1) only moderate inhibition was found for these compounds. In order to obtain transitio