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The synthesis of oligodeoxyprimidines on a polymer support

✍ Scribed by M.D. Matteucci; M.H. Caruthers

Publisher
Elsevier Science
Year
1980
Tongue
French
Weight
274 KB
Volume
21
Category
Article
ISSN
0040-4039

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✦ Synopsis

A polymer supported method for synthesizing deoxypolynucleotides is described . Two nonanucleotides were synthesized . Yields exceeding 90% were obtained for each condensation . The time per nucleotide addition was four hours .

Attempts to develop a successful methodology for synthesizing sequence defined oligonucleotides have been numerous l ' 2 . However despite these achievements the stepwise synthesis of oligonucleotides still remains a difficult and time consuming task . We are developing procedures which should solve this problem . This communication outlines our initial results on adapting the phosphite coupling procedure 3 to polymer supported polynucleotide synthesis .

Classically a major problem with polymer supported synthetic strategies has been the polymer support . Various polymers have been shown to be inadequate for reasons such as slow diffusion rates into the support, excessive swelling of various macroporous, low crosslinked polymers and

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