✦ LIBER ✦

The subcellular localization of newly synthesized cytochrome b5.

✍ Scribed by Jens Carlsen; Kirsten Christiansen

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 514 KB
Volume: 19
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.1995.1127

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✦ Synopsis

Abstract

The fate of newly synthesized cytochrome b~5~ was studied in rat hepatocytes. Using an antibody specific for microsomal cytochrome b~5~, we found newly synthesized microsomal cytochrome b~5~ in both mitochondria and a mitochondria associated membrane fraction as well as in microsomes. Newly synthesized cytochrome b~5~ was quickly removed from the site of synthesis on free ribosomes and inserted into membranes at random. No migration of newly synthesized cytochrome b~5~ between cellular compartments was observed and therefore the assembly of the apoprotein with the heme moiety is apparently not taking place in any particular cellular compartment.

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