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A simple procedure for the solubilization of NADH-cytochrome b5 reductase

✍ Scribed by Arun C. Dey; Sheilagh Rahal; Robert L. Rimsay; Ian R. Senciall

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
502 KB
Volume
110
Category
Article
ISSN
0003-2697

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✦ Synopsis

NADH-cytochrome

b, reductase has been solubilized by extraction of rabbit liver microsomes with 1 M potassium phosphate buffer (pH 7.4), and has been purified to comparable purity with the Triton X-1OOsolubilized enzyme. Gel electrophoresis indicated an apparent molecular weight of 33,000 for both phosphate buffer-extracted and Triton X-100~solubilized enzymes. Phosphate buffer extraction provides a simple mild procedure for the extraction of NADH-cytochrome b, reductase that avoids detergents or proteolytic agents.

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