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The structure of the exopolysaccharide from Rhizobium sp. strain ANU280 (NGR234)

✍ Scribed by Steven P. Djordjevic; Barry G. Rolfe; Michael Batley; John W. Redmond

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
805 KB
Volume
148
Category
Article
ISSN
0008-6215

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✦ Synopsis

The structure of an exopolysaccharide from Rhizobium sp. strain ANU280 (derivative of the broad host-range strain NGR234) has been determined. Fragments generated by partial acid hydrolysis were fractionated by sequential ionexchange and gel chromatography, and their structures were assigned by 13C-n.m.r. spectroscopy. Extensive overlap of structure between the fragments, together with the results of periodate oxidation and calorimetric analyses, permitted assignment of the nonasaccharide repeating-unit shown below. The terminal galactosyl group carries a 4,6-O-(l-carboxyethylidene) group and, probably, an acetyl group.

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