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The Structure of Lipid A of the Lipopolysaccharide from Burkholderia caryophylli with a 4-Amino-4-deoxy-L-arabinopyranose 1-Phosphate Residue Exclusively in Glycosidic Linkage

✍ Scribed by Antonio Molinaro; Buko Lindner; Cristina De Castro; Birte Nolting; Alba Silipo; Rosa Lanzetta; Michelangelo Parrilli; Otto Holst

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 275 KB
Volume: 9
Category: Article
ISSN: 0947-6539
DOI: 10.1002/chem.200390177

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✦ Synopsis

From the lipopolysaccharides (LPSs) of the plant-pathogenic bacterium Burkholderia caryophylli, the complete structure of lipid A has been characterized. For the first time, a 4-amino-4-deoxy-L-arabinopyranose 1-phosphate residue was proven to be exclusively linked to the reducing end of lipid A from a wild-type LPS. The LPSs of B. caryophylli were degraded by mild acetate buffer hydrolysis at pH 4.4. The obtained lipid A was analyzed as such, and also after de-O-acylation or dephosphorylation. The structure of lipid A was identified mainly by means of matrix-assisted laser desorption/ionisation mass spectrometry, and by various 1D and 2D (1)H and (13)C NMR spectroscopic measurements.

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✍ Evgeny Vinogradov; Buko Lindner; Guntram Seltmann; Joanna Radziejewska-Lebrecht; 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB

## Abstract The carbohydrate backbones of the core‐lipid A region were characterized from the lipopolysaccharides (LPSs) of __Serratia marcescens__ strains 111R (a rough mutant strain of serotype O29) and IFO 3735 (a smooth strain not serologically characterized but possessing the O‐chain structure