Lipopolysaccharides from Serratia marcescens Possess One or Two 4-Amino-4-deoxy-L-arabinopyranose 1-Phosphate Residues in the Lipid A and D-glycero-D-talo-Oct-2-ulopyranosonic Acid in the Inner Core Region
✍ Scribed by Evgeny Vinogradov; Buko Lindner; Guntram Seltmann; Joanna Radziejewska-Lebrecht; Otto Holst
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 164 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0947-6539
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✦ Synopsis
Abstract
The carbohydrate backbones of the core‐lipid A region were characterized from the lipopolysaccharides (LPSs) of Serratia marcescens strains 111R (a rough mutant strain of serotype O29) and IFO 3735 (a smooth strain not serologically characterized but possessing the O‐chain structure of serotype O19). The LPSs were degraded either by mild hydrazinolysis (de‐O‐acylation) and hot 4 M KOH (de‐N‐acylation), or by hydrolysis in 2 % aqueous acetic acid, or by deamination. Oligosaccharide phosphates were isolated by high‐performance anion‐exchange chromatography. Through the use of compositional analysis, electrospray ionization Fourier transform mass spectrometry, and ^1^H and ^13^C NMR spectroscopy applying various one‐ and two‐dimensional experiments, we identified the structures of the carbohydrate backbones that contained D‐glycero‐D‐talo‐oct‐2‐ulopyranosonic acid and 4‐amino‐4‐deoxy‐L‐arabinose 1‐phosphate residues. We also identified some truncated structures for both strains. All sugars were D‐configured pyranoses and α‐linked, except where stated otherwise.