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The structure of human thyroglobulin: Sedimentation equilibrium in 6 M guanidine hydrochloride

โœ Scribed by A.B. Rawitch; D.M. Brown


Book ID
115749465
Publisher
Elsevier Science
Year
1973
Weight
466 KB
Volume
295
Category
Article
ISSN
0005-2795

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A technique has been perfected for measuring the sedimentation coefficient of microgram quantities of a reduced protein in 6 M guanidine hydrochloride. The protein is sedimented through a gradient of 5-8 M guanidine-HCl in the presence of dithiothreitol in a SW 50.1 swinging-bucket rotor. Run condit