A method for the measurement of the sedi
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Bruce W. Patterson; Verne N. Schumaker; Waldo R. Fisher
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Article
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1984
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Elsevier Science
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English
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A technique has been perfected for measuring the sedimentation coefficient of microgram quantities of a reduced protein in 6 M guanidine hydrochloride. The protein is sedimented through a gradient of 5-8 M guanidine-HCl in the presence of dithiothreitol in a SW 50.1 swinging-bucket rotor. Run condit