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Determination of molecular weight of proteins and protein subunits in the presence of 6M guanidine hydrochloride

✍ Scribed by Ullmann, Agnes.; Goldberg, Michel E.; Perrin, David.; Monod, Jacques.

Book ID
126811552
Publisher
American Chemical Society
Year
1968
Tongue
English
Weight
435 KB
Volume
7
Category
Article
ISSN
0006-2960

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A technique has been perfected for measuring the sedimentation coefficient of microgram quantities of a reduced protein in 6 M guanidine hydrochloride. The protein is sedimented through a gradient of 5-8 M guanidine-HCl in the presence of dithiothreitol in a SW 50.1 swinging-bucket rotor. Run condit