The Structural Gene for λ Exonuclease
✍ Scribed by Charles M. Radding, Josiane Szpirer and René Thomas
- Book ID
- 123647819
- Publisher
- National Academy of Sciences
- Year
- 1967
- Tongue
- English
- Weight
- 828 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/57945
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A specialized transducing bacteriophage lambdadpolCdap D-9 has been isolated that carries the structural gene for EF-Ts1 (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UVL-inactivated cells has been detected by two-dimensional gel electrophoresis and
Genes that are preferentially expressed in a particular developmental pathway can be isolated by subtractive hybridization (SH). We developed a PCRbased approach coupled with exonuclease digestion that allows for generating single-stranded tester and driver nucleic acids suitable for SH starting fro