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A transducing bacteriophage λ carrying the structural gene for elongation factor Ts

✍ Scribed by Friesen, James D. ;Parker, Jack ;Watson, Robert J. ;Bendiak, Dirk ;Reeh, Solvejg V. ;Pedersen, Steen ;Fiil, Niels P.

Book ID
104693642
Publisher
Springer
Year
1976
Tongue
English
Weight
528 KB
Volume
148
Category
Article
ISSN
0026-8925

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✦ Synopsis

A specialized transducing bacteriophage lambdadpolCdap D-9 has been isolated that carries the structural gene for EF-Ts1 (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UVL-inactivated cells has been detected by two-dimensional gel electrophoresis and has been verified by antibody precipitation. In an induced lysogen of this phage the relative rate of synthesis of EF-Ts is increased 4-fold. Evidence is presented which suggest that the structural genes for ribosomal protein S2 (rpsB) and RNA polymerase sigma factor (sit) also lies on lambdadpolCdap D-9.

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