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Isolation of a transducing phage carrying rpsT, the structural gene for ribosomal protein S20

✍ Scribed by Friesen, James D. ;Parker, Jack ;Watson, Robert J. ;Fiil, Niels P. ;Pedersen, Steen

Publisher
Springer
Year
1976
Tongue
English
Weight
312 KB
Volume
144
Category
Article
ISSN
0026-8925

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✦ Synopsis

Lambda transducing phages carrying segments of the Escherichia coli chromosome in the dapB region have been isolated in their in vivo gene products analyzed by two-dimensional gel electrophoresis. One of these phages, lambdaddapB-2, carries the structural genes for ribosomal protein S20 (rps T) and isoleucyltransfer RNA synthetase (ileS.) The most likely gene order is thr-rpsT-ileS-dapB-pyrA.

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Identity of a gene responsible for suppr
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✍ Buckel, Peter πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 368 KB

Sepcialized transducing lines of phage lambda carrying segments between thr and car from the E. coli chromosome have been isolated. With help of these phages it has been shown that the gene sups20 (BΓΆck et al., 1974) corresponds to rpsT, the structural gene for ribosomal protein S20.

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