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The stimulation of DNA synthesis by cytochalasin B in proliferative epidermal and dermal cells

✍ Scribed by Simon Rothberg; Grace E. Nancarrow; Virgene L. Church

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
650 KB
Volume
95
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Cytochalasin B influences a variety of cellular events that are associated with the contractile microfilament system and the formation of binucleate cells. Along with the formation of binucleate cells, cytochalasin B also causes an acceleration of cells from G~1~ to S in the cell cycle

By pulsing with cytochalasin B for 30 minutes and allowing for a previously established lag time (17.5 hours) a stimulation of thymidine incorporation into DNA of proliferative epidermal and dermal cells was found in both control and stripped epidermis

Autoradiographic analysis confirmed that the stimulation was due to an increased number of basal cells accelerated from G~1~ to S phase. A minimal number of binucleate basal cells, 1 in 300, was observed, which suggests that the stimulated synthesis is independent of binucleate cell formation. The amount of stimulation is maximum with cytochalasin B concentration pulse between 5Ξ³ and 30Ξ³/ml

The results suggest a possible link in coupling cell membrane and surface events with subsequent increased cell nuclei synthetic activity.

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