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The Stereochemistry of Hydrogen Transfer from NADPH Catalysed by 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase from Rat Liver

โœ Scribed by Alan S. Beedle; Kenneth A. Munday; David C. Wilton


Book ID
115113195
Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
444 KB
Volume
28
Category
Article
ISSN
1432-1327

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A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2