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Some properties of purified 3-hydroxy-3-methylglutaryl coenzyme A reductase phosphatases from rat liver

โœ Scribed by Gregorio Gil; Fausto G. Hegardt


Book ID
115705098
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
592 KB
Volume
214
Category
Article
ISSN
0003-9861

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A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2