The stabilizing effect of soluble macromolecules on enzyme performance

✍ Scribed by Liliana Gianfreda; Guido Greco

• Publisher: Springer Netherlands
• Year: 1981
• Tongue: English
• Weight: 290 KB
• Volume: 3
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00127257

✦ Synopsis

A new enzyme stabilization method is proposed which is associated with dynamical immobilization within an unstirred ultrafiltration membrane reactor. Upon injection of suitable amounts of macromolecular solutions, the very polarization phenomena that yield the immobilization produce high macromolecular concentration levels in the reactor region immediately upstream from the ultrafiltration membrane where the enzymes operate. This procedure results in considerable improvements in en zyme stability that seem to be quite independent of the nature of the stabilizing macromolecule adopted. Stabilization effects of the same order of magnitude have been obtained for different enzymatic systems.