The spread of plasmids as a function of bacterial adaptability
✍ Scribed by H. Tschäpe
- Book ID
- 109301872
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 581 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0168-6496
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A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15–40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca
When hamster cells are infected with the bacterial plasmid colicinogenic factor E1 (ColE1), as much as 5-8% of the input plasmid radioactivity is found in the recipient cell, mainly in the nuclear fraction. Density shift experiments with bromodeoxyuridine labeled ColE1 DNA indicate that part of the