The small, versatilepPZPfamily ofAgrobacteriumbinary vectors for plant transformation

✍ Scribed by Peter Hajdukiewicz; Zora Svab; Pal Maliga

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 445 KB
• Volume: 25
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00014672

✦ Synopsis

The new pPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322 bom site for mobilization from Escherichia coli to Agrobacterium, and the ColE1 and pVS 1 plasmid origins for replication in E. coli and in Agrobacterium, respectively. Bacterial marker genes in the vectors confer resistance to chloramphenicol (pPZP100 series) or spectinomycin (pPZP200 series), allowing their use in Agrobacterium strains with different drug resistance markers. Plant marker genes in the binary vectors confer resistance to kana- mycin or to gentamycin, and are adjacent to the left border (LB) of the transferred region. A lacZ ~-peptide, with the pUC 18 multiple cloning site (MCS), lies between the plant marker gene and the right border (RB). Since the RB is transferred first, drug resistance is obtained only if the passenger gene is present in the transgenic plants.