𝔖 Bobbio Scriptorium
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Next generation synthetic vectors for transformation of the plastid genome of higher plants

✍ Scribed by Sugey Ramona Sinagawa-García; Tarinee Tungsuchat-Huang; Octavio Paredes-López; Pal Maliga

Publisher
Springer
Year
2009
Tongue
English
Weight
535 KB
Volume
70
Category
Article
ISSN
0167-4412

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The loxP-Cre site-specific recombination system of phage P1 was used to develop a novel strategy to construct cointegrate vectors for Agrobacterium-mediated plant transformation. A pTi disarmed helper plasmid (pAL1166) was constructed by replacing the oncogenic T-DNA by a loxP sequence and a spectin