The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination
โ Scribed by Xie, Jianming; Wang, Lei; Wu, Ning; Brock, Ansgar; Spraggon, Glen; Schultz, Peter G
- Book ID
- 109908296
- Publisher
- Nature Publishing Group
- Year
- 2004
- Tongue
- English
- Weight
- 310 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1013
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๐ SIMILAR VOLUMES
## Abstract An __Escherichia coli__ suppressor tRNA^Phe^ (tRNA^Phe^~CUA~) was misacylated with 4โiodoโLโphenylalanine by using the A294G phenylalanylโtRNA synthetase mutant (G294โPheRS) from __E. coli__ at a high magnesiumโion concentration. The preacylated tRNA was added to an __E. coli__ cellโfre
Procedures for a radiometric assay of L-[guanidinooxy-14C]canavanine were developed which provide a convenient and accurate measure of the incorporation of [14C]canavanine into de novo-synthesized proteins. These methods are also applicable to determining [14C]arginine incorporation into protein. Th