✦ LIBER ✦
A radiometric assay for determining the incorporation of l-canavanine or l-arginine into protein
✍ Scribed by Gerald A. Rosenthal; Deborah A. Thomas
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 362 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Procedures for a radiometric assay of L-[guanidinooxy-14C]canavanine were developed which provide a convenient and accurate measure of the incorporation of [14C]canavanine into de novo-synthesized proteins. These methods are also applicable to determining [14C]arginine incorporation into protein. These procedures have been employed to study the synthesis of L-[guanidinooxy-14C]canavanine- and L-[guanidino-14C]arginine-containing proteins from the hemolymph of Manduca sexta and Heliothis virescens, two highly destructive insect pests.