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The significance of serum mitochondrial aspartate aminotransferase activity in the surgical field

✍ Scribed by Eishin Sada; Seiki Tashiro; Yoshimasa Morino

Book ID
112755779
Publisher
Springer
Year
1991
Tongue
English
Weight
726 KB
Volume
21
Category
Article
ISSN
1436-2813

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The optimal conditions for selective proteolytic inactivation of cytosolic aspartate aminotransferase (c-AST) to determine mitochondrial aspartate aminotransferase (m-AST) in serum were studied. Protease 401 was found to be effective over a pH range of 6.0-10.0. A pH of 9.5 with 0.5% albumin in the