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The separation of α- and β-rabbit globin mRNA by polyacrylamide gel electrophoresis

✍ Scribed by Anton Berns; Peter Jansen; Hans Bloemendal

Book ID
115904969
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
325 KB
Volume
47
Category
Article
ISSN
0014-5793

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✍ T.Gordon Wood; Joseph R. Shaeffer 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 361 KB

A procedure to separate the (Y and /3 globin chains of rabbit hemoglobin. denatured with sodium dodecyl sulfate in the presence of mercaptoethanol. on a column of polyacrylamide gel was developed. The identity of the two separated chains was verified by (a) differences in distribution of radioactivi