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Separation of the α- and β-Chains of Globins by Means of Starch-gel Electrophoresis

✍ Scribed by MULLER, C. J.

Book ID
109608321
Publisher
Nature Publishing Group
Year
1960
Tongue
English
Weight
119 KB
Volume
186
Category
Article
ISSN
0028-0836

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Separation of alpha and beta chains of r
Separation of alpha and beta chains of rabbit globin using SDS-polyacrylamide gel electrophoresis
✍ T.Gordon Wood; Joseph R. Shaeffer 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 361 KB

A procedure to separate the (Y and /3 globin chains of rabbit hemoglobin. denatured with sodium dodecyl sulfate in the presence of mercaptoethanol. on a column of polyacrylamide gel was developed. The identity of the two separated chains was verified by (a) differences in distribution of radioactivi